M-08: Improvement of Intestinal Dysbiosis With Exogenous Prebiotic Metabolites Reduces Intestinal Bowel Inflammation

Poster Presenter

Millicent Yeboah-Awudzi

Graduate Assistant and PhD Student
Louisiana State University
United States

Objectives

To determine the efficacy of proanthocyanidin B-2, butyrate, propionate, phytic acid, or phosvitin phosphopeptide on gliadin-induced inflammation in Caco-2 as a model of intestinal dysbiosis

Method

Caco-2 was incubated with 20 µg/ml of gliadin peptide 31-43 in absence or presence of proanthocyanidin B-2, butyrate, phytic acid, phosvitin at 37 °C, 5% CO2 for 24 h. Cell supernatants were analyzed for TGAse, IL-15, IL-6, IL-8, and NF-KB. Tight junctions were evaluated by E-cadherin and TEER.

Results

Gliadin peptide 31-34 disrupted tight junctions and enhanced inflammatory TGAse, IL-15, IL-6, IL-8, and NF-KB. Proanthocyanidin, phytic acid and phosvitin phosphopeptides at micromolar, and butyrate or propionate at 2 mM inhibited all the inflammatory markers. All these bioactive compounds improved tight junctions through higher transepithelial electric resistance (TEER) and E-cadherin levels.

Conclusion

Normalizing gut dysbiosis through microbial implantation has been identified as a new approach to overcome diseases of insulin resistance, such as diabetes mellitus, celiac disease, and depressive disorders. But normalizing gut dysbiosis is costly, hence the direct ingestion of naturally occurring prebiotic metabolites or phenolic anthocyanins showed in this study inhibits a wide range of inflammatory biomarkers associated with dysbiosis and may be a good alternative to gut microbiota normalization or fecal microbiota implantation. However, in vivo studies are needed to corroborate these in vitro results.